Cytotoxicity tests of extracts from white kwao krua, pueraria mirifica, red kwao krua, betea superba and black kwao krua, mucuna collettii on Hep-G2 cells

To evaluate the proliferative and antiproliferative effect in vitro of the plant crude extracts (white Kwao Krua, Pueraria mirifica, red Kwao Krua, Butea superba and black Kwao Krua, Mucuna collettii) to human hepatoblastoma, Hep-G2 cell line. The cells were incubated with the 4 plant extracts at the concentration of 1, 10, 100 and 1000 µg/ml for 3 days and assay for the percent of cells viability by MTT compared with the control (P < 0.05). Samples of P. mirifica extracts collected from Nan (1000 µg/ml) and Ratchaburi provinces (1 µg/ml) exhibited most proliferative and anti-proliferative effect, respectively. Samples of B. superba extracts collected from Chaing Mai (1000 µg/ml) and Khon Kaen provinces (1000 µg/ml) exhibited most proliferative and antiproliferative effect, respectively. Samples of M. collettii extracts collected from Kanchanaburi (1000 µg/ml) and Chaing Mai provinces (1000 µg/ml) exhibited most proliferative and anti-proliferative effect, respectively. Very interesting, proliferative effect to Hep-G2 itself may refer to rejuvenating effect of the plant extracts as normally the hepatocytes can do rapid cell division if the hepatic tissue is damaged. Besides, the antiproliferative effect to the cells may enable establishment of study to develop the plant product into a therapeutic medicine for cancer cells.