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Sensitive Superoxide Biosensor Based on Silicon Carbide Nanoparticles
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AbstractDirect electron transfer of immobilized superoxide dismutase (Cu, Zn‐SOD) onto silicon carbide (SiC) nanoparticles displays a pair of well defined and nearly reversible redox peaks with formal potential (E°′) of −0.03 V in pH 7.4. The heterogeneous electron transfer rate constant (ks) and surface coverage (Γ) of immobilized SOD are 11.0±0.4 s−1 and 1.42×10−11 mol cm−2. Biosensor shows fast amperometric response (3s) with sensitivity and detection limit of 1.416 nA μM−1, 1.66 μM, and 1.375 nA μM−1, 2.1 μM for cathodically or anodically detection of superoxide, respectively. This biosensor also exhibits good stability, reproducibility and long life‐time.
Title: Sensitive Superoxide Biosensor Based on Silicon Carbide Nanoparticles
Description:
AbstractDirect electron transfer of immobilized superoxide dismutase (Cu, Zn‐SOD) onto silicon carbide (SiC) nanoparticles displays a pair of well defined and nearly reversible redox peaks with formal potential (E°′) of −0.
03 V in pH 7.
4.
The heterogeneous electron transfer rate constant (ks) and surface coverage (Γ) of immobilized SOD are 11.
0±0.
4 s−1 and 1.
42×10−11 mol cm−2.
Biosensor shows fast amperometric response (3s) with sensitivity and detection limit of 1.
416 nA μM−1, 1.
66 μM, and 1.
375 nA μM−1, 2.
1 μM for cathodically or anodically detection of superoxide, respectively.
This biosensor also exhibits good stability, reproducibility and long life‐time.
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