O-GlcNAc Regulated Proliferation and Deterioration of the Nasal Inverted Papilloma

Abstract Background: The nasal inverted papilloma occurs mostly in the epithelium of the nasal mucosa. Histopathological manifestations of the nasal inverted papilloma are benign but it has the characteristics of aggressive growth, strong local destruction, frequent recurrence, and malignant change. Nasal inverted papilloma is a tumor with malignant biological behavior. O-GLcNAc is a posttranslational modification that is ubiquitous in cells. This seemingly simple carbohydrate modification played a key role in cell physiology and disease progression. Methods: In this study, immunohistochemical staining and western blot were used to determine the expression of O-GlcNAc in the nasal inverted papilloma; RT-qPCR was used to detect the expression of ogt. The expression levels of ogt and oga genes were detected by RT-qPCR in the SCC6 and CNE-E1 cells. An ogt and oga small-interference RNA fragment was transfected into cells to both reduce and increase the O-GlcNAc. The effect of O-GlcNAc on the proliferative ability of cells was detected by CCK8. The migration and invasion of cells was detected by wound healing assay and transwell invasion assay. Results: The expression of O-GlcNAc and ogt mRNA levels in nasal inverted papilloma were higher than that in the control group. O-GlcNAc enhanced SCC6- and CNE-E1- cell proliferative, migratory, and invasive ability. This study found that changes in the glycosylation level of O-GLcNAc affected the proliferation, invasion, and migration of the NIP.