Trypan blue and Turk solution v1

Trypan Blue test Trypan is test used as a stain to selectively colour dead cells. Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.Trypan is test used as a stain to selectively colour dead cells. Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method. Türk's solution Türk's solution is a hematological stain (crystal violet or aqueous methylene blue) that destroys the membrane of red blood cells (RBCs), white blood cells (WBCs) and platelets and platelets within a blood sample, and stains the nuclei of the white blood cells, making them easier to see and count. Turk's solution is a gold standard for counting leukocytes in a defined volume of blood.