Use Immunohistochemistry to Determine the Severity of CD34 Expression in Psoriasis that has been Histopathologically Diagnosed

Objective: The goal of this study is to determine the degree of CD4 expression in histopathologically confirmed cases of psoriasis using immunohistochemistry. Study Design: Cross-sectional/ Descriptive study Place and Duration: Pathology Department of North West School of Medicine (NWSM), Peshawar and Avicenna Medical and Dental College, Lahore for duration of six months April 2021 to September 2021. Methods: There were 80 patients of both genders with age 18-70 years were presented in this study. Informed written consent was taken from all the patients for detailed demographics including age, sex and body mass index. Psoriasis was diagnosed by taking skin biopsy of all the patients. Histopathology slides were made from paraffin-embedded sections of the whole skin biopsy. When examined in the 40x magnification field, the cases were classified as having light staining (4-10 capillaries), moderate staining (11-20 capillaries), or strong staining (21-28 capillaries). On the basis of histopathology, psoriasis was determined to be present when the following features were observed: hyperkeratosis, acanthosis, a munro's abscess, the extension of rete ridges, and abnormalities of the dermal vasculature. SPSS 24.0 version was used to analyze. Results: Majority of the patients in or study were males 55 (68.8%) and the rest were females 25 (31.2%) with mean age 35.51+19.61 years. Mean BMI of the patients was 24.23+11.34 kg/m2. Frequency of moderate staining of CD34 expression was high found in 50 (62.5%) cases, mild staining in 17 (21.3%) patients and 21-28 capillaries (strong staining) in 13 (16.3%) patients. According to histopathological findings hyperkeratosis, acanthosis, a munro's abscess, the extension of rete ridges, and abnormalities of the dermal vasculature majority had moderate (11-20 capillaries) staining of CD34 expression. Conclusion: We concluded in this study that the mild staining expression of CD34 was majority seen among patients of psoriasis. Psoriasis's enhanced cutaneous vasculature has been demonstrated by this crucial angiogenesis marker. As a result, it can be used to better understand the disease's histiogenesis. Keywords: CD34 expression, Histopathology, Immunohistochemistry, Psoriasis