Anti-Inflammatory Activity Assay Using the Human Red Blood Cell Membrane Stabilization Method for Pasote Leaf Extract (Dysphania ambrosioides L.)

Dysphania ambrosioides L., or pasote leaves, is a herbal plant widely used in traditional medicine in various parts of the world, including in Indonesia. This plant is known to have multiple properties, especially in overcoming health problems related to inflammation. Inflammation is a complex biological response to injury or infection. Therefore, searching for compounds that can reduce inflammation is very important in developing new therapies. A useful technique for assessing a compound's anti-inflammatory properties is the red blood cell membrane stabilization method. This technique measures the harm that inflammatory agents cause to the membrane of red blood cells. This study aims to evaluate the anti-inflammatory activity of pasote leaf extract using the red blood cell membrane stabilization method. This study used a post-test-only control design method and a pure experimental research design. This research was conducted at the Pharmacy Laboratory, Faculty of Mathematics and Natural Sciences (FMIPA). The sample used was rat blood taken through the retroorbital sinus of the eye. The criteria for selecting rats were rats weighing 200-250 grams and healthy as indicated by active movement. Purposive sampling separated the samples into seven groups. The number of subjects in this study was 35 samples. Diclofenac sodium is a non-steroidal anti-inflammatory drug used to reduce inflammation and pain. At the highest concentration tested, which was 250 ppm, sodium diclofenac showed very high inhibitory activity with an average of 92.57%. Pasote leaf extract (Dysphania ambrosioides L.) is known to have various pharmacological potentials, including anti-inflammatory activity. At the highest concentration tested, which was 250 ppm, the pasote leaf extract showed very high inhibitory activity with an average of 78.72%. The erythrocyte membrane stabilization test results demonstrated the strong anti-inflammatory properties of pasote leaf extract, as the percentage ...