QUANTITATIVE DETERMINATION OF FLAVONOIDS IN E. ANGUSTFOLIUM RAW MATERIALS BY SPECTROPHOTOMETRIC METHOD AND METHOD VALIDATION

The purpose of the work is to determine the amount of flavonoids in the grass E. angustfoli-um using spectrophotometry and assess the validity of the method. The raw material of the species E. angustifolium used for research purposes was collected in the flowering phase of the plant from the Guba region of the Republic of Azerbaijan in July 2022. The validation parameters of the method for spectrophotometric quantification of flavonoids in the E. angustifolium herb were assessed in accordance with the requirements of international documents. The amount of flavonoids in the E.angustifolium herb was determined using a spectrophoto-metric method and it was found that the amount of flavonoids was ± 2.77%. A validation assessment of the spectrophotometric method for the quantitative determination of flavonoids in E.angustifolium raw materials was carried out, the reliability interval of the method accuracy indicator was 0.56; linearity index in the concentration range of 0.01-0.05 mg/ml (R2=0.999); coefficient of variation of the accuracy indicator - 0.26% on the first day, 0.31% on the second day; The limit of determination of the method was found to be 0.02 mg/ml, and the limit of determination of quantity was found to be 0.006 mg/ml. The conducted studies show that the herb E.angustifolium is quite rich in flavonoids, which lays the foundation for expanding the local medicinal plant raw material base in the future.