Biobanking of Biological Samples at Room Temperature

Abstract Biospecimens have become an integral part of life science research, pharma and drug development. There is increased demand for biospecimens from patients with assured quality of collection, processing, and storage. Establishing a biobank with high-quality biospecimens is an expensive aspect due to the requirement of costly freezers and liquid nitrogen facilities. Many studies have explored various storage reagents for biological samples. Still, the need of the hour is the one that can preserve biological samples at ambient room temperature and suitable for diagnosis and research. InstaPRESERVE is an eco/user-friendly solution with long-term sustainable alternative for freezer or liquid nitrogen systems for biobanking. Blood and saliva specimens were collected and stored in InstaPRESERVE at room temperature and conventional storage conditions. The genomics and proteomics analysis of the DNA, RNA, and protein extracted from the -80°C and InstaPRESERVE stored specimens and fresh specimens indicated that the specimens were well-conserved at room temperature compared to frozen specimens. Innovative technologies and approaches are being evaluated in biobanks for sample integrity, reducing collection, processing, and storage costs, increasing speed and throughput, and reducing energy expenditure. Preserving the biospecimens in InstaPRESERVE at room temperature eliminates expensive storage systems, cold-chain during transportation, and pre-processing requirements. The qualitative and quantitative results of DNA, RNA, and protein ensured stabilized storage for a longer period. The use of InstaPRESERVE as a specimen storage medium is emerging as an alternative in biobanking for specimen storage at room temperature. The existing and proposed biobanks may consider employing InstaPRESERVE as a reliable resource for biospecimens storage.