Influence of triphenyltetradecylphosphonium bromide on the Na+-Li+- countertransport rate in the erythrocyte membrane in patients with genetically different permeability of cell membranes to sodium

Aim. To study the influence of triphenyltetradecylphosphonium bromide [(PPh)3P+C14H29] Br- on the cell membranes permeability to Na+ by determining the rate of Na+-Li+-counter transport in erythrocyte membrane depending on it’s variable initial condition. Methods. Blood samples of 10 healthy volunteers with different Na+-Li+-counter transport rate distribution in erythrocyte membrane were analyzed: I quartile (5 subjects) - low permeability, III quartile (5 subjects) - moderately high permeability. Results. Na+-Li+-counter transport rate change in erythrocyte membrane under the influence of triphenyltetradecylphosphonium bromide depends on genetically determined initial membrane permeability. In subjects belonging to III quartile of Na+-Li+-counter transport the initial membrane permeability is high, and after administration of 0.001 and 0.005 μM concentrations of [(PPh)3P+C14H29] Br- membrane permeability to Na+ increased. In subjects belonging to I quartile of Na+-Li+-counter transport (low initial permeability) [(PPh)3P+C14H29] Br- did not alter membrane permeability to Na+. Conclusion. Triphenyltetradecylphosphonium bromide increases erythrocyte membrane permeability to Na+ in subjects belonging to III quartile of Na+-Li+-counter transport and dose not influence subjects belonging to I quartile of Na+-Li+-counter transport.