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Modern Methods to Explore GPCR Signalling in Live Cells
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Investigating the intricate mechanisms of G protein-coupled receptor
(GPCR) signalling in living cells is far from trivial. Over the last 20
years, the rise of genetically encoded resonance energy transfer (RET)
sensors has shed new light onto the mechanisms of GPCR signalling. Such
findings have challenged classical views on GPCR signalling and enhanced
our understanding of the spatiotemporal dimensions of GPCR activity,
leading to the discovery of endosomal GPCR signalling. This review
highlights the use of RET sensors to monitor GPCR signalling in
real-time and in live cells, focusing on GPCR activation and
trafficking, and second messenger activity. It explores the
physiological relevance of illustrative cases of endosomal signalling
and discusses potential avenues to improve RET approaches to further
explore endogenous GPCR activity in physiologically relevant contexts.
Title: Modern Methods to Explore GPCR Signalling in Live Cells
Description:
Investigating the intricate mechanisms of G protein-coupled receptor
(GPCR) signalling in living cells is far from trivial.
Over the last 20
years, the rise of genetically encoded resonance energy transfer (RET)
sensors has shed new light onto the mechanisms of GPCR signalling.
Such
findings have challenged classical views on GPCR signalling and enhanced
our understanding of the spatiotemporal dimensions of GPCR activity,
leading to the discovery of endosomal GPCR signalling.
This review
highlights the use of RET sensors to monitor GPCR signalling in
real-time and in live cells, focusing on GPCR activation and
trafficking, and second messenger activity.
It explores the
physiological relevance of illustrative cases of endosomal signalling
and discusses potential avenues to improve RET approaches to further
explore endogenous GPCR activity in physiologically relevant contexts.
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