In vitro evaluation of tooth whitening potential of peroxide-free OTC dental bleaching agents

Abstract Purpose: To evaluate and compare the tooth whitening potential of five over-the-counter (OTC) peroxide-free dental bleaching, as well as an experimental tooth whitening solution containing 0,1% hydrogen peroxide complexed with doping agents with a gold standard (positive control) containing 16% carbamide peroxide. Material and methods: Eighty permanent bovine incisor teeth were randomly allocated to eight different groups. Two teeth from each group were immerged into five staining solution represented by coffee, tea, red wine, curry mixed in warm oil or distilled water (control group) and stored at 37°C during 28 days in an incubator. The teeth were then reallocated to the eight groups, resulting in ten samples per group, and each group was matched with a bleaching product. The bleaching procedures were executed following the manufacturer’s recommendations. The color of each sample was assessed over a white and black background using a quantitative numerical measurement approach with a calibrated spectrophotometer. Spectrophotometric measurements were performed after exposing the teeth to the bleaching agent for 60 min (T2), 100 min (T3), 200 min (T4), and ΔE00 was calculated. Results: When analysed over a white background, the mean ΔE00 values ranged from 2.14 (Placebo) to 6.32 (Opalescence PF). When analysed over a black background, the mean ΔE00 values ranged from 2.31 (Placebo) to 5.78 (Opalescence PF). Statistically significant ΔE00 color changes over time for the eight groups and five staining solutions at T1 and T4 were assessed for both background using repeated ANOVA followed by Fisher's LSD post-hoc test (p-value < 0.01). Conclusion: All tested over-the-counter whitening kits except one, exhibited positive color variation. However, the individual performance differed vastly from one brand to the other and the overall performance was less effective compared to conventional carbamide peroxide-based positive control.