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Expression, purification, and characterization of VPS13C v1
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VPS13C is a ER-lysosome contact site protein that is thought to transport lipids between the two organellar membranes and important for lysosomal membrane damage repair. We employed structure-function analysis of purified VPS13C and uncovered novel regulatory mechanisms. This protocol details the method for expressing and purifying the human full-length VPS13C from Expi293F cells as well as structural characterization by negative-stain electron microscopy (EM). Procedures were similarly applied to the expression, purification, and in vitro characterization of other VPS13C mutants.
Title: Expression, purification, and characterization of VPS13C v1
Description:
VPS13C is a ER-lysosome contact site protein that is thought to transport lipids between the two organellar membranes and important for lysosomal membrane damage repair.
We employed structure-function analysis of purified VPS13C and uncovered novel regulatory mechanisms.
This protocol details the method for expressing and purifying the human full-length VPS13C from Expi293F cells as well as structural characterization by negative-stain electron microscopy (EM).
Procedures were similarly applied to the expression, purification, and in vitro characterization of other VPS13C mutants.
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