Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Simultaneous determination of Cefepime and Tazobactam by using Hyphenated Liquid Chromatography (UPLC-MS/MS)
View through CrossRef
The present study deals with establishing a hyphenated analytical method and validating the same for the determination of the drug combination of Cefepime and Tazobactam simultaneously by UPLC-MS/MS. A C18 BEH analytical column was employed. A mobile phase of 0.2 percent formic acid in water and acetonitrile were used in a 20:80 v/v ratio. Samples are ionized using +ESI ionization and detected in MRM mode. The retention time of 0.80 min and 0.96 min were observed for Cefepime and Tazobactam respectively. The drugs have linear responses in the investigated range, with r2 = 0.9955 for Cefepime and r2 = 0.9816 for Tazobactam with increased precision and accuracy. The LOD for Cefepime and Tazobactam was 66ng/ml and 8.3ng/ml respectively. The LOQ for Cefepime and Tazobactam was 200ng/ml and 25ng/ml respectively. According to the findings, the hyphenated UPLC-MS/MS method was developed and validated as per ICH regulations to determine Cefepime and Tazobactam simultaneously.
Title: Simultaneous determination of Cefepime and Tazobactam by using Hyphenated Liquid Chromatography (UPLC-MS/MS)
Description:
The present study deals with establishing a hyphenated analytical method and validating the same for the determination of the drug combination of Cefepime and Tazobactam simultaneously by UPLC-MS/MS.
A C18 BEH analytical column was employed.
A mobile phase of 0.
2 percent formic acid in water and acetonitrile were used in a 20:80 v/v ratio.
Samples are ionized using +ESI ionization and detected in MRM mode.
The retention time of 0.
80 min and 0.
96 min were observed for Cefepime and Tazobactam respectively.
The drugs have linear responses in the investigated range, with r2 = 0.
9955 for Cefepime and r2 = 0.
9816 for Tazobactam with increased precision and accuracy.
The LOD for Cefepime and Tazobactam was 66ng/ml and 8.
3ng/ml respectively.
The LOQ for Cefepime and Tazobactam was 200ng/ml and 25ng/ml respectively.
According to the findings, the hyphenated UPLC-MS/MS method was developed and validated as per ICH regulations to determine Cefepime and Tazobactam simultaneously.
Related Results
Neurotoxic effects of a well known antibiotic!
Neurotoxic effects of a well known antibiotic!
To the Editor,
Cefepime is a fourth-generation cephalosporin with broad-spectrum antimicrobial activity covering a wide range of both gram-negative and gram positive organisms. It ...
Risk factors for cefepime nonsusceptible Gram-negative infections in allogeneic hematopoietic cell transplant recipients
Risk factors for cefepime nonsusceptible Gram-negative infections in allogeneic hematopoietic cell transplant recipients
Purpose Allogeneic hematopoietic cell transplant recipients undergo myelosuppressive chemotherapy to allow engraftment of stem cells and are at particularly high risk for bacterial...
Hippocampal Concentrations Drive Seizures in a Rat Model for Cefepime-induced Neurotoxicity
Hippocampal Concentrations Drive Seizures in a Rat Model for Cefepime-induced Neurotoxicity
BackgroundIn high dose, cefepime causes neurotoxicity in patients with kidney injury; however, the relationship between exposure and observed neurotoxicity is not clear, and no ani...
Clinical Pharmacology of Piperacillin/Tazobactam
Clinical Pharmacology of Piperacillin/Tazobactam
Piperacillin extends the spectrum of activity of ampicillin to include most strains of Pseudomonas aeruginosa, Enterobacteriaceae (non-β-lactamase-producing), many Bacteroides spec...
Antibiotic Cefepime with Antacid, Metal Complexation studied by UV Spectrophotometrically in addition to Exploration of Antimicrobial Activity, In-Vitro Demonstration
Antibiotic Cefepime with Antacid, Metal Complexation studied by UV Spectrophotometrically in addition to Exploration of Antimicrobial Activity, In-Vitro Demonstration
As a fourth generation of cephalosporins, cefepime shows very superior activity in opposition to gram - negative organisms like Pseudomonas aeruginosa in addition to gram – positiv...
A meta-analysis of piperacillin/tazobactam versus cefepime treatment in cancer patients with febrile neutropenia
A meta-analysis of piperacillin/tazobactam versus cefepime treatment in cancer patients with febrile neutropenia
Febrile neutropenia (FN) causes a major threat to cancer patients after chemotherapy. Broad-spectrum antibiotic treatment is a well-established practice for febrile neutropenia. Pi...
Challenging Management of Postoperative Empyema: A Case Report with Literature Review
Challenging Management of Postoperative Empyema: A Case Report with Literature Review
Abstract
Introduction: Pleural empyema is the collection of pus within the pleural cavity, typically arising as a complication of pneumonia, chest trauma, thoracic surgery, or bact...
Smaller but more frequent dosing of cefepime in the treatment of febrile neutropenia
Smaller but more frequent dosing of cefepime in the treatment of febrile neutropenia
Background
Generally national guidelines for febrile neutropenia recommend treatment with cefepime 2 g every 8 h. Due to beta-lactam’s time dependent killing ef...