The effect of hydrolase enzyme on antibiofilm activity in oral cavity microbes

Background : Every surface of the human body that comes into contact with the outside environment is covered with a layer of microorganisms called the human microbiome, characterized by species diversity and a high number of cells. Biofilms are surface-related, three-dimensional multicellular structures whose integrity depends on the extracellular matrix produced by their constituent bacteria. Hydrolase enzymes are a class of enzymes belonging to the anti-biofilm enzymes and are categorized as polysaccharide-degrading enzymes, proteolytic enzymes, and antiquorum sensing enzymes. Purpose : To know the effect of hydrolase enzyme towards antibiofilm activity of oral cavity microbe. Method : The method used is literature study by searching databases from various national and international sources. Results : There is an anti-biofilm effect of several types of hydrolase enzymes on oral microbes such as P. aeruginosa, S. aureus, E. faecalis, and E. coli. The greatest effect was found in the enzymes PelAh & PslGh with the mechanism of action of polysaccharide-degrading enzymes and their microbial target, P. aeruginosa, can reduce biofilm biomass by 58-94%. Conclusion : Of the several existing mechanisms can provide different effects depending on the microbial target.