Single-neuron and population measures of neuronal activity in working memory tasks

ABSTRACT Information represented in working memory is reflected in the firing rate of neurons in the prefrontal cortex and brain areas connected to it. In recent years, there has been an increased realization that population measures capture more accurately neural correlates of cognitive functions. We examined how single neuron firing in the prefrontal and posterior parietal cortex of two male monkeys compared with population measures in spatial working memory tasks. Persistent activity was observed in the dorsolateral prefrontal and posterior parietal cortex and firing rate predicted working memory behavior, particularly in the prefrontal cortex. These findings had equivalents in population measures, including trajectories in state space that became less separated in error trials. We additionally observed rotations of the stimulus space for different task conditions, which was not obvious in firing rate measures. These results suggest that population measures provide a richer view of how neuronal activity is associated with behavior, however, largely confirm that persistent activity is the core phenomenon that maintains visual-spatial information in working memory. NEW & NOTEWORTHY Recordings from large numbers of neurons led to a re-evaluation of neural correlates of cognitive functions, which traditionally were defined based on responses of single neurons, or averages of firing rates. Analysis of neuronal recordings from the dorsolateral prefrontal and posterior parietal cortex revealed that properties of neuronal firing captured in classical studies of persistent activity can account for population representations, though some population characteristics did not have clear correlates in single neuron activity.