Ziqi Dihuang decoction ameliorates thrombosis in septic rats by inhitbiting plasminogen activator inhibitor-1

AbstractBackground and AimSepsis is now a global medical burden with high morbility and mortality. The focus of this study was to observe and elaborate the effect of Ziqi Dihuang (ZQDH) decoction on inflammatory and thrombosis-related parameters in septic rats.Experimental procedureA rat model of sepsis was established by cecal ligation and puncture (CLP). 24 male Sprague-Dawley (SD) rats were randomly divided into Sham group, CLP group, ZQDH-1ow group (0.735g/kg) and ZQDH-high group (1.47g/kg). Rats in ZQDH groups were given ZQDH decoction by gavage for 7 days before CLP, while rats in Sham and CLP groups were given the same amount of normal saline. Leukocytes and percentage of neutrophils (N %) in blood, and inflammatory cell infiltration of liver, kidney and lung were used to assess systemic inflammatory response. Coagulation and fibrinolytic indexes included platelet count, coagulation function, fibrin deposition, and levels of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) in serum, liver, kidney and lung.ResultsZQDH decoctioninhibited N% growth in blood and inflammatory cell infiltration in the lung of CLP rats. Moreover, ZQDH decoctionalso ameliorated thrombocytopenia and prothrombin time, alleviated renal fibrin deposition, and improved tPA and PAI-1 levels in kidney.ConclusionThese results suggest that ZQDH decoction can dose-dependently relieve systemic inflammatory injury and regulate coagulation-fibrinolysis system in septic rats, which may be mediated by PAI-1.Highlights of the findings and noveltiesZQDH decoction relieves systemic inflammatory injury in sepsis;ZQDH decoction regulates coagulation-fibrinolysis system in sepsis;ZQDH decoction inhibits fibrinolysis by reducing inhibitor-1 (PAI-1) level in sepsis.