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Neutral α-mannosidase from fungus Aspergillus fischeri (NCIM 508) belongs to Class IIC subfamily
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BACKGROUD & OBJECTIVE: The α-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNAmethod. METHODOLOGY: Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene wascarried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology.RESULTS:The α-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showedpresence of three introns and four exons in the gene. BLAST analysis revealed 100% similarity to the Neosartoryafischeri (A. fischeri) and 94% with Aspergillus fumigatus class I α-mannosidase reported in NCBI Gene Data Bank.CONCLUSION: The neutral α-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily.
Maharani Lakshmi Ammanni College for Women
Title: Neutral α-mannosidase from fungus Aspergillus fischeri (NCIM 508) belongs to Class IIC subfamily
Description:
BACKGROUD & OBJECTIVE: The α-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNAmethod.
METHODOLOGY: Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene wascarried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology.
RESULTS:The α-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showedpresence of three introns and four exons in the gene.
BLAST analysis revealed 100% similarity to the Neosartoryafischeri (A.
fischeri) and 94% with Aspergillus fumigatus class I α-mannosidase reported in NCBI Gene Data Bank.
CONCLUSION: The neutral α-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily.
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