SEMEN QUALITY OF HORNED AND POLLED BALI BULLS TREATED WITH BEAN SPROUT (Phaseolus radiatus L.) SUPPLEMENTED FEEDING

<p><strong>Background</strong>. Horned and Polled Bali cattle are big meat producing livestock and have various other advantages, so they need to be maintained regarding their existence by improving semen quality. Providing bean sprouts (<em>Phaseolus radiatus L.</em>) in feeding is an effort to improve semen quality because of its antioxidant content that can scavenge free radicals. <strong>Objective.</strong> To determine the quality of semen from Bali Polled bull and Bali Horned bull fed with bean sprout feed supplements. <strong>Methodology.</strong> Four Horned and four Polled Bali bulls aged 5-7 years were fed bean sprouts-supplemented twice a day in the amount of 1 kg/bull for two months. Parameters observed in fresh semen include color, smell, pH, volume, concentration, and followed by motility, viability, abnormality, membrane integrity, and acrosome integrity for both fresh and frozen semen. <strong>Results.</strong> The results of the macroscopic evaluation of fresh semen showed that the color, smell, pH, and volume were not different (P&gt;0.05) in Polled compared to Horned. Meanwhile, the microscopic evaluation showed that motility, viability, abnormality, membrane integrity, and acrosome integrity were not different (P&gt;0.05) between Horned and Polled, but the sperm concentration of Horned was higher (P&lt;0.05) than Polled (1.160 ± 1.16 x 10⁹ vs 0.840 ± 0.92 x 10⁹). The quality of frozen semen showed that the motility and membrane integrity of Polled is higher (P&lt;0.05) than Horned (48.57 ± 3.77% vs. 35.38 ± 12.15% and 76.60 ± 4.30% vs. 70.00 ± 5.79%), whereas recovery rate was higher (P&lt;0.01) of Polled than Horned (69.39 ± 3.88% vs. 50.54 ± 9.02%), but in the parameters of viability, abnormality, and acrosome integrity were not different (P&gt;0.05). <strong>Implications</strong>. This information from this study can be used to improve semen the quality of Horned and Polled Bali bulls fed bean sprouts. <strong>Conclusion.</strong> In conclusion, after bean sprouts (<em>Phaseolus radiatus L.) </em>supplemented feeding, the semen concentration of Horned increases compared to the Polled one. While the color, smell, pH, volume, motility, viability, abnormality of membrane integrity, and acrosome integrity of fresh semen were not affected. The motility, recovery rate, and membrane integrity of frozen semen are increased in Polled compared to the Horned one, while viability, abnormalities, and acrosome integrity remained similar.</p><strong></strong>