Abstract 1163: Chemoprevention of testosterone-induced benign prostatic hyperplasia by hexane fraction from seeds of Annona muricata (soursop) in rats

Abstract Alternative medicine is an extensively recognized therapeutic approach for the management of several diseases. Annona muricata commonly called sour sop is traditionally used to manage an array of human diseases, especially cancer. In this study, we hypothesized that hexane fraction from seeds of Annona muricata (HFAM) will ameliorate testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) in rat model. Castration was performed via the scrotal route under ketamine anesthesia and BPH was induced through daily intraperitoneal injection of TP (3 mg/kg) for 4 weeks after castration. Rats were assigned into six groups of six animals each viz: non-castrated control, castrated control, castrated rats that received TP (BPH group), BPH + HFAM, BPH + HFAM + finasteride, BPH + finasteride. Results showed that BPH rats had 3.1 and 2.4 folds increase in prostatic and organo-somatic weight, respectively. Furthermore, BPH increased the activities of total acid and alkaline phosphatases by 62% and 53%, respectively, and levels of lipid peroxidation products by 48% in the prostate of rats. Also, BPH caused significant increase in the levels of inflammatory markers (Myeloperoxidase and total nitric oxide) in rats. In contrast, there were no significant differences in the activities of prostatic antioxidant enzymes in BPH group relative to others. In BPH group, immuno-histochemistry revealed strong expression of prostatic inducible nitric oxide synthase, Bcl2, beta-catenin, androgen and estrogen receptors proteins, while mild expression of p53 and Bax were observed. Interestingly, treatment of BPH rats with HFAM or HFAM + finasteride significantly attenuated inflammatory indices and induced apoptotic parameters. Overall, hexane fraction from seeds of Annona muricata may be used as a therapeutic agent for BPH via anti-inflammatory effect. Citation Format: Oluwatosin A. Adaramoye, Taiwo D. Oladipo, Olubukola O. Akanni, Solomon O. Owumi, Olusoji J. Abiola. Chemoprevention of testosterone-induced benign prostatic hyperplasia by hexane fraction from seeds of Annona muricata (soursop) in rats [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1163.