Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Organization of 5S ribosomal DNA of Litchi chinensis Sonn.
View through CrossRef
Aim. 5S ribosomal DNA (5S rDNA) represents a universal model for studying the evolution of repeated sequences in eukaryotic organisms. Taking into account that this region of the genome still remains almost undescribed in species of the family Sapindaceae, we investigated the molecular organization of a repeated unit of 5S rDNA in a member of this family, Litchi chinensis. Methods. PCR amplification, cloning and sequencing of 5S rDNA. Results. It was found that the length of the repeated unit of the 5S rDNA of L. chinensis is 321 -323 bp. The level of intragenomic similarity of 5S rDNA repeats is 87.1 %. Potential external elements of the RNA polymerase III promoter, which are localized in IGS, differ from those described for members of other families of angiosperms. Conclusions. In the genome of L. chinensis, at least two classes of 5S rDNA repeats are present, which differ in the sequence of external promoter elements.
Keywords: 5S rDNA, molecular evolution, Litchi chinensis, Sapindaceae.
Institute of Molecular Biology and Genetics (NAS Ukraine)
Title: Organization of 5S ribosomal DNA of Litchi chinensis Sonn.
Description:
Aim.
5S ribosomal DNA (5S rDNA) represents a universal model for studying the evolution of repeated sequences in eukaryotic organisms.
Taking into account that this region of the genome still remains almost undescribed in species of the family Sapindaceae, we investigated the molecular organization of a repeated unit of 5S rDNA in a member of this family, Litchi chinensis.
Methods.
PCR amplification, cloning and sequencing of 5S rDNA.
Results.
It was found that the length of the repeated unit of the 5S rDNA of L.
chinensis is 321 -323 bp.
The level of intragenomic similarity of 5S rDNA repeats is 87.
1 %.
Potential external elements of the RNA polymerase III promoter, which are localized in IGS, differ from those described for members of other families of angiosperms.
Conclusions.
In the genome of L.
chinensis, at least two classes of 5S rDNA repeats are present, which differ in the sequence of external promoter elements.
Keywords: 5S rDNA, molecular evolution, Litchi chinensis, Sapindaceae.
Related Results
Knowledge of the Members of Agnigarh Producer Company Limited towards Improved Litchi Cultivation Practices in Sonitpur District of Assam, India
Knowledge of the Members of Agnigarh Producer Company Limited towards Improved Litchi Cultivation Practices in Sonitpur District of Assam, India
Farmer Producer Company is a company formed by a group of farmers who are producers come together to form a company. The present investigation was conducted out with the objective ...
Evaluation of Soil Physicochemical Parameters across Selected Litchi (Litchi chinensis Sonn.) Cultivars
Evaluation of Soil Physicochemical Parameters across Selected Litchi (Litchi chinensis Sonn.) Cultivars
Litchi (Litchi chinensis Sonn.) is a member of the Sapindaceae family and is occasionally referred to as the “Queen of Fruits.” The exceptional flavor, scent, white aril, and eye-c...
Impact of different treatments to enhance the fruit quality of litchi (Litchi chinensis Sonn.) cv. Bombai under stored temperature
Impact of different treatments to enhance the fruit quality of litchi (Litchi chinensis Sonn.) cv. Bombai under stored temperature
Litchi (Litchi chinensis) is a delicious and juicy fruit. In India, it is cherished for its flavourful aril and consumed as a fresh table fruit, whereas, in China and Japan, it is ...
Effects of Litchi Production on Rural Household Income in District Charsadda, Khyber Pakhtunkhwa
Effects of Litchi Production on Rural Household Income in District Charsadda, Khyber Pakhtunkhwa
Purpose: This study was examine the effects of litchi production and key socio-economic factors on the household income of rural farmers in District Charsadda, Khyber Pakhtunkhwa.
...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Abstract
Background: Age-associated epigenetic alteration is the underlying cause of DNA damage in aging cells. Two types of youth-associated DNA-protection epigenetic mark...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Introduction: The United States currently faces two opioid crises, an evolved crisis currently manifesting as widespread abuse of illicit opioids, and a crisis in pain management l...
Lipid Profiling Leymus chinensis Root Insensitive to Ca Deprivation
Lipid Profiling Leymus chinensis Root Insensitive to Ca Deprivation
Abstract
Background
Leymus chinensis (L. chinensis) is a perennial native forage grass widely distributed in the steppe of Inner Monoglia as the dominant species. Calcium ...
Origin, history, production and processing.
Origin, history, production and processing.
AbstractThis chapter covers the history of cultivation of longan and litchi (Litchi chinensis); litchi production in China, Vietnam, Thailand, India, Bangladesh, Nepal, Philippines...