EVALUATION THE RATE OF DELAYED PARASITE CLEARANCE IN UNCOMPLICATED PLASMODIUM FALCIPARUM PATIENT AFTER 3 DAYS TREATMENT WITH DIHYDROARTEMISININ PLUS PIPERAQUIN PHOSPHATE (DHA-PPQ) IN HUONG HOA DISTRICT, QUANG TRI PROVINCE

Background: Malaria is still remains a public health disease with high circulating levels, each year causes the death of about 1.5 million people worldwide, and almost all deaths are caused by falciparum malaria. The emergence of multidrug-resistant P. falciparum parasites has made these drugs useless in many areas where malaria is endemic. As with earlier antimalarial drugs,parasite resistance to artemisinin and its derivatives has emerged in Southeast Asia. Evaluate the effectiveness of antimalarial drug is an essential activity for assessing sensitivity of drug resistance are taking a positive contribution to the prevention of malaria. Research objective: To evaluate the rate of delayed parasite clearance (DPC) in uncomplicated Plasmodium falciparum patient after 3 days treatment with DHA-PPQ in Huong Hoa district, Quang Tri province. Research methods: Cross-sectional studyandempirical research. The research samples included all patients with falciparum malaria uncomplicated in Huong Hoa district, Quang Tri province agreed to participate in our study. There are 84 patients were collected smears and whole blood samples before and after 3 days of treatment with DHA-PPQ for evaluating parasitemiae by microscopy method and Real time PCR. Results: In total 84 samples of malaria patients were collected to determine the rate of delayed parasite clearance (DPC) after 3 days of treatment with DHA-PPQ, there were 22 samples (26.2%) are parasites in blood after treatment 3days determined by microscopy; Real time PCR with 33 samples (39.3%) were parasites in the blood after treatment 3 days. Conclusion: The epidemiology of malaria in our study had a rate of delayed parasite clearance (DPC)after treatment with DHA-PPQ 3 days were 26.2% (22/84 samples) determined by microscopy and 39.3% (33/84 samples) by Real time PCR. As defined by WHO, the study results contribute to the initial report suspected artemisinin resistance occurs in endemic areas of our research. Key words: Plasmodium falciparum, Artemisinin.