Physiological Effects of Lactic Acid Bacteria on Induced Non-Alcoholic Fatty Liver Disease in Male Laboratory Rats

Abstract To better understand the causes and effects of non-alcoholic fatty liver disease and to help find effective treatments for it, the animal model is seen as an alternate model. For this investigation, researchers utilised adult male rats of the Sprague dawely strain that were albino (Rattus norvegicus) and had an age range of 2-3 months. Their mass ranged from 170 to 200 grammes. Two axes were used to divide the experiment. There were two categories on the first axis, which represented the infection stage. Five animals served as a healthy control group in the first set. The first group consumed regular food and water for forty-five days, whereas the second group consumed animal fats. Combine with the meal at a rate of thirty percent of the diet’s weight (HF), for fifteen animals over the course of forty-five days. Both groups had blood biochemical testing once the infectious stage came to a close. At the end of the infection phase, blood biochemical tests were performed for both groups. In comparison to the healthy control group, the study found that glucose, total cholesterol (TCH), triglycerides (TG), low-density lipoproteins (LDL), very low-density lipoproteins (VLDL), and malondialdehyde (MDA) levels were significantly higher. In contrast, high-density lipoproteins (HDL) and glutathione (GSH) concentrations were significantly lower. Sugar, total cholesterol, triglycerides, low-density lipoproteins, very low-density lipoproteins, and malondialdehyde were measured during the 28-day therapy period. Alternatively, there was a significant decrease in levels of glutathione (GSH) and high-density lipoproteins (HDL). While contrasted with the infected control group. The lactic acid bacteria (L. plantarum) and lactic acid bacteria (L. rhamnose) caused significant weight loss in animals when administered in a dosage. Infected control rats (HF) that were treated with these bacterial groups while still eating a high-fat diet showed significant improvements in liver function and blood parameters, such as lower percentages of total cholesterol (TCH), triglycerides (TG), and low-density lipoproteins (LDL). There was an uptick in both HDL and very high-density lipoprotein (VLDL). In comparison to the afflicted control groups, the therapy groups demonstrated a marked recovery while simultaneously lowering oxidative stress factor levels.