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CLEC-2 suppresses calcification in cultured osteoblasts
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Abstract
Podoplanin is the only counter-receptor of platelet CLEC-2 and is expressing on mature osteoblast, but there is no report on the role of podoplanin and CLEC-2 in calcification. This study aimed to investigate the role of podoplanin binding to CLEC-2 in the calcification of osteoblasts carrying homozygously deleted
Pdpn
alleles (
Pdpn
Δ/Δ
) by heterozygously expressing collagen type I alpha 1 promoter (
Col1a
)-driven
Cre
recombinase. There were no macroscopic abnormalities in the bone and dentin of
Col1a11-Cre;Pdpn
Δ/Δ
mice but the coccygeal bone medullary cavity was very narrow. In the quantitative analysis for alizarin red-stained products and alkaline phosphatase activities on the cultured calvarial osteoblasts, the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts of both
Pdpn
fl/fl
and
Col1a11-Cre;Pdpn
Δ/Δ
mice were significantly higher in the calcification medium than in the α-mem. Both the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts from
Pdpn
fl/fl
mice were significantly lower in the calcification medium with CLEC-2 than without CLEC-2 while there were no significant differences in the amounts of calcified products and alkaline phosphatase activities of calvarial osteoblasts from
Col1a11-Cre;Pdpn
Δ/Δ
mice with CLEC-2. Platelet CLEC-2 may play a role in regulating the calcification via binding to podoplanin on mature osteoblasts expressing podoplanin in the medullary cavity of a part of the bone.
Title: CLEC-2 suppresses calcification in cultured osteoblasts
Description:
Abstract
Podoplanin is the only counter-receptor of platelet CLEC-2 and is expressing on mature osteoblast, but there is no report on the role of podoplanin and CLEC-2 in calcification.
This study aimed to investigate the role of podoplanin binding to CLEC-2 in the calcification of osteoblasts carrying homozygously deleted
Pdpn
alleles (
Pdpn
Δ/Δ
) by heterozygously expressing collagen type I alpha 1 promoter (
Col1a
)-driven
Cre
recombinase.
There were no macroscopic abnormalities in the bone and dentin of
Col1a11-Cre;Pdpn
Δ/Δ
mice but the coccygeal bone medullary cavity was very narrow.
In the quantitative analysis for alizarin red-stained products and alkaline phosphatase activities on the cultured calvarial osteoblasts, the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts of both
Pdpn
fl/fl
and
Col1a11-Cre;Pdpn
Δ/Δ
mice were significantly higher in the calcification medium than in the α-mem.
Both the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts from
Pdpn
fl/fl
mice were significantly lower in the calcification medium with CLEC-2 than without CLEC-2 while there were no significant differences in the amounts of calcified products and alkaline phosphatase activities of calvarial osteoblasts from
Col1a11-Cre;Pdpn
Δ/Δ
mice with CLEC-2.
Platelet CLEC-2 may play a role in regulating the calcification via binding to podoplanin on mature osteoblasts expressing podoplanin in the medullary cavity of a part of the bone.
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