Testicular Ultrastructure of Zebu Bulls in Costa Rica

SummaryThe testicular ultrastructure of sexually mature Zebu (Bos indicus) bulls from Costa Rica was studied in relation to their classification based on andrological examinations undertaken in the field. Based on the testicular consistency at palpation, each bull was assigned to one of three groups, ranging from normal (Group A) to markedly reduced testicular consistency (Group C). The ultrastructure of the clinically normal testicle (Group A) in the Zebu bull resembled that of Bos taurus. In testicles with a slight to moderate reduction of testicular consistency (Group B) degenerated primary spermatocytes and spermatids were common at all stages of the seminiferous cycle. Abnormalities in the condensation of the chromatin, acrosomal defects, and the presence of degenerated cells and cellular debris were the most common of disturbances found. In testicles from bulls with a marked reduction of testicular consistency (Group C) the seminiferous epithelium of most tubules showed degenerative changes, thereby making it impossible to classify the stage of the cycle. The changes were mainly restricted to spermatocytes and spermatids, whose relative numbers were reduced. The number of cells undergoing meiotic divisions was also greatly reduced. Changes in the spermatids were also found including the appearance of severe acrosomal defects, such as acrosomal pouches, the failure of nuclear elongation and clear defects in chromatin condensation. The present ultrastructural findings in Bos indicus do not differ from the picture of normal testicular structure and of testicular degeneration usually found in Bos taurus bulls.