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Validated LC–MS/MS method for quantification of agomelatine in human plasma and its application in a pharmacokinetic study
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An analytical method based on liquid–liquid extraction has been developed and validated for analysis of agomelatine in human plasma. Fluoxetine was used as an internal standard for agomelatine. A Betasil C18 (4.0 × 100 mm, 5 µm) column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic conditions and mass spectrometric detection in the positive ionization mode using an API‐4000 system. The proposed method has been validated with linear range of 0.050–8.000 ng/ml for agomelatine. The intra‐run and inter‐run precision values are within 12.12% and 9.01%, respectively, for agomelatine at the lower limit of quantification level. The overall recovery for agomelatine and fluoxetine was 67.10% and 72.96%, respectively. This validated method was used successfully for analysis of plasma samples from a pharmacokinetic study. Copyright © 2012 John Wiley & Sons, Ltd.
Title: Validated LC–MS/MS method for quantification of agomelatine in human plasma and its application in a pharmacokinetic study
Description:
An analytical method based on liquid–liquid extraction has been developed and validated for analysis of agomelatine in human plasma.
Fluoxetine was used as an internal standard for agomelatine.
A Betasil C18 (4.
0 × 100 mm, 5 µm) column provided chromatographic separation of analytes followed by detection with mass spectrometry.
The method involves simple isocratic chromatographic conditions and mass spectrometric detection in the positive ionization mode using an API‐4000 system.
The proposed method has been validated with linear range of 0.
050–8.
000 ng/ml for agomelatine.
The intra‐run and inter‐run precision values are within 12.
12% and 9.
01%, respectively, for agomelatine at the lower limit of quantification level.
The overall recovery for agomelatine and fluoxetine was 67.
10% and 72.
96%, respectively.
This validated method was used successfully for analysis of plasma samples from a pharmacokinetic study.
Copyright © 2012 John Wiley & Sons, Ltd.
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