Next-generation sequencing with emphasis on Illumina and Ion torrent platforms.

Abstract Background: Next-generation sequencing is a type of deep sequencing. In comparison to the previously used Sanger's method, Next generation sequencing allowing the sequencing of an entire genome in a single day. Next-generation sequencing (NGS) has revolutionized genomics and molecular biology. NGS has a wide range of medical applications, including tumors and inherited disease diagnosis. It is also used to find genetic variants across the genome. There are several NGS platforms available. Illumina and Iontorrent are the most prevalent sequencing platforms. These NGS platforms reduced the cost and time required to sequence a full genome. The main body of the abstract: The review paper covered a brief history of next-generation sequencing technology (NGS), followed by the benefits of employing this revolutionary technology and The general approach of NGS, beginning with fragmentation and ending with data analysis, was explained, with a focus on the Illumina and Ion torrent platforms. Finally, the data analysis step was thoroughly covered, beginning with data quality control and ending with data visualization. Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications. Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications.