Development of High Performance Liquid Chromatographic Estimation of Domperidone and Lansoprazole

The combination of Domperidone and Lansoprazole is very useful in Gastro-esophageal disinfection (Dyspepsia). These methods provide means to separate the components characterize and quantify the components. An accurate, precise, specific and simple HPLC method was developed for simultaneous estimation of Domperidone and Lansoprazole. By this method retention time, linearity and accuracy data is respectively found for Domperidone and Lansoprazole. Mobile phase was prepared by mixing 51 volume of Acetonitrile and 49 volume of Ammonium Acetate (51:49 V/V) then 25mg each of Domperidone and Lansoprazole was dissolved in small volume of Acetonitrile: Ammonium Acetate (51:49 V/V) separately. Retention time was recorded 4.330 ± 0.003 minute and 5.820 ± 0.003 minute for Domperidone and Lansoprazole with 1.0 ml/min flow rate. The low value of % R.S.D indicates that this method is precise and accurate. Thus it can be concluded that the proposed method was good approach for obtaining reliable result.