Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Prevalence, Molecular Characterization, Pathogenicity and Antimicrobial Susceptibility Pattern of Clostridioides Difficile From Clinical Samples in South Eastern Nigeria.
View through CrossRef
Abstract
The prevalence, molecular characterization, pathogenicity and antibioticsensitivity pattern of Clostridioides difficile from clinical samples in South-Eastern Nigeria wereevaluated in this study. Stool samples (450) were collected from patientsinsome selected public and private-owned clinics in the five South-Eastern states. Standard bacteriological methods were employed for enumeration of total anaerobes. Culture of Clostridioides difficile isolates were performed on Clostridium difficile differential agar (supplemented with C. difficile selective supplement). Biochemical tests like catalase, oxidase and reverse CAMP test were conducted before extraction of genomic bacterial DNA for suspected C. difficile isolates. Purification and amplification of bacterial DNA was carried out on 2% agarose gel. Amplified bacteria DNA was sequenced and blasted on the National Centre for Biotechnology Information (NCBI) website. Antibiotics susceptibility was carried out for C. difficile isolates using the Kirby-Bauer disc diffusion technique. PCR technique was employed for the detection of virulence (tcdA, tcdB, cdtAandcdtB) and resistance genes (tetS, tetAandermB) in C. difficile isolates. Anaerobe counts for stool samples obtained showed that samples had a mean count of 5.63±0.09 log10CFU/g (Anambra state) while counts of 5.61±0.11 log10CFU/g and 5.65±0.07 log10CFU/g were obtained from Ebonyi and Enugu States. The antibacterial sensitivity revealed that the isolates were sensitive to gentamicin, meropenem and amoxicillin-clavulanic acid. The isolates were resistant to tetracyclines and erythromycin, and the multiple antibiotic resistance index of theisolates showed that the multi-drug resistant isolates had a MAR index of 0.44. All (14 C. difficile isolates) (100%) of C. difficile isolates were found to possess tcdBgenes, and 69.56% expressed tcdAgenes. One (4.34%) of the C. difficile isolatespossessed cdtAand cdtBgenes for binary toxin production.
Springer Science and Business Media LLC
Title: Prevalence, Molecular Characterization, Pathogenicity and Antimicrobial Susceptibility Pattern of Clostridioides Difficile From Clinical Samples in South Eastern Nigeria.
Description:
Abstract
The prevalence, molecular characterization, pathogenicity and antibioticsensitivity pattern of Clostridioides difficile from clinical samples in South-Eastern Nigeria wereevaluated in this study.
Stool samples (450) were collected from patientsinsome selected public and private-owned clinics in the five South-Eastern states.
Standard bacteriological methods were employed for enumeration of total anaerobes.
Culture of Clostridioides difficile isolates were performed on Clostridium difficile differential agar (supplemented with C.
difficile selective supplement).
Biochemical tests like catalase, oxidase and reverse CAMP test were conducted before extraction of genomic bacterial DNA for suspected C.
difficile isolates.
Purification and amplification of bacterial DNA was carried out on 2% agarose gel.
Amplified bacteria DNA was sequenced and blasted on the National Centre for Biotechnology Information (NCBI) website.
Antibiotics susceptibility was carried out for C.
difficile isolates using the Kirby-Bauer disc diffusion technique.
PCR technique was employed for the detection of virulence (tcdA, tcdB, cdtAandcdtB) and resistance genes (tetS, tetAandermB) in C.
difficile isolates.
Anaerobe counts for stool samples obtained showed that samples had a mean count of 5.
63±0.
09 log10CFU/g (Anambra state) while counts of 5.
61±0.
11 log10CFU/g and 5.
65±0.
07 log10CFU/g were obtained from Ebonyi and Enugu States.
The antibacterial sensitivity revealed that the isolates were sensitive to gentamicin, meropenem and amoxicillin-clavulanic acid.
The isolates were resistant to tetracyclines and erythromycin, and the multiple antibiotic resistance index of theisolates showed that the multi-drug resistant isolates had a MAR index of 0.
44.
All (14 C.
difficile isolates) (100%) of C.
difficile isolates were found to possess tcdBgenes, and 69.
56% expressed tcdAgenes.
One (4.
34%) of the C.
difficile isolatespossessed cdtAand cdtBgenes for binary toxin production.
Related Results
Potable Water Sources, Household Hygiene, and Sanitation Practices in Ikpoba Okha LGA, Edo State: Implications for Public Health and Sustainable Water Management
Omoregie, Andrew Edosa.1 Omoregie Abieyuwa Peace2 Okoro, Enyinnaya Okoro.3
1 College of Medi
Potable Water Sources, Household Hygiene, and Sanitation Practices in Ikpoba Okha LGA, Edo State: Implications for Public Health and Sustainable Water Management
Omoregie, Andrew Edosa.1 Omoregie Abieyuwa Peace2 Okoro, Enyinnaya Okoro.3
1 College of Medi
BACKGROUND
Access to potable drinking water and sufficient sanitation continues to be an urgent global concern, particularly in developing regions where con...
Evolution of Antimicrobial Resistance in Community vs. Hospital-Acquired Infections
Evolution of Antimicrobial Resistance in Community vs. Hospital-Acquired Infections
Abstract
Introduction
Hospitals are high-risk environments for infections. Despite the global recognition of these pathogens, few studies compare microorganisms from community-acqu...
Direct and culture-independent detection of low-abundant
Clostridioides difficile
in environmental DNA via PCR
Direct and culture-independent detection of low-abundant
Clostridioides difficile
in environmental DNA via PCR
Abstract
Clostridioides difficile
represents a major burden to public health. As a well-known nosocomial pathogen whose occurre...
Phenotypic and Molecular Characterization of the blaTEM Gene in Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae
Phenotypic and Molecular Characterization of the blaTEM Gene in Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae
Abstract
Introduction
There has been a notable rise in antibiotic resistance among enterobacteria. This issue is primarily attributed to the emergence of extended-spectrum beta-lac...
Butyrate enhances Clostridioides difficile sporulation
in vitro
Butyrate enhances Clostridioides difficile sporulation
in vitro
ABSTRACT
Short chain fatty acids (SCFAs) are products of bacterial fermentation that help maintain important gut functions such as the intestinal...
Health care associated Clostridioidesdifficile infection and colonization in patients admitted at tertiary care hospital Pakistan
Health care associated Clostridioidesdifficile infection and colonization in patients admitted at tertiary care hospital Pakistan
Objective: To evaluate the epidemiology of clostridioides difficile infections and colonisation in a tertiary-care setting.
Method: The cross-sectional study was conducted at the C...
Poziom wiedzy personelu pielęgniarskiego na temat Clostridioides difficile
Poziom wiedzy personelu pielęgniarskiego na temat Clostridioides difficile
Wystąpienie zakażenia wywołanego przez Clostridioides difficile wymaga od personelu pielęgniarskiego szybkiej reakcji polegającej na wdrożeniu postępowania przeciwepidemicznego, wp...
Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017
Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017
AbstractClostridioides difficile PCR ribotype (RT) 017 ranks among the most successful strains of C. difficile in the world. In the past three decades, it has caused outbreaks on f...