Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Screening for L1014F mutation inB. rufimanus v1
View through CrossRef
This protocol describes the process of screening the broad bean bruchid beetle, Bruchus rufimanus (Coleoptera; Chrysomelidae) for the L1014F mutation that has been associated with insecticide resistance in other Coleoptera species (and more widely across insect species). The starting point is a quick and high-throughout non-destructive DNA isolation from beetles, followed by amplification of a region of the Voltage-gated sodium channel gene (VGSC) that harbours the L1014F mutation. The resulting PCR products can be sent for Sanger sequencing to confirm presence/absence of the L1014F mutation.
Springer Science and Business Media LLC
Title: Screening for L1014F mutation inB. rufimanus v1
Description:
This protocol describes the process of screening the broad bean bruchid beetle, Bruchus rufimanus (Coleoptera; Chrysomelidae) for the L1014F mutation that has been associated with insecticide resistance in other Coleoptera species (and more widely across insect species).
The starting point is a quick and high-throughout non-destructive DNA isolation from beetles, followed by amplification of a region of the Voltage-gated sodium channel gene (VGSC) that harbours the L1014F mutation.
The resulting PCR products can be sent for Sanger sequencing to confirm presence/absence of the L1014F mutation.
Related Results
Resistance status of Anopheles gambiae (s.l.) to four commonly used insecticides for malaria vector control in South-East Nigeria
Resistance status of Anopheles gambiae (s.l.) to four commonly used insecticides for malaria vector control in South-East Nigeria
Abstract
Background
Progress made in the control of malaria vectors globally is largely due to the use of insecticides. However, success in the figh...
Meta-analysis of economic evaluation studies: data harmonisation and methodological issues
Meta-analysis of economic evaluation studies: data harmonisation and methodological issues
AbstractBackgroundIn the context of ever-growing health expenditure and limited resources, economic evaluations aid in making evidence-informed policy decisions. Cost-utility analy...
A Study on the Therapeutic Ability of Gut Microbiota upon <i>V. parahaemolyticus </i>Infected Farm Prawn
A Study on the Therapeutic Ability of Gut Microbiota upon <i>V. parahaemolyticus </i>Infected Farm Prawn
Glycogen content of the prawn muscles from the three groups showed increasing trend after 30 days of feed supplementation. In control animals recorded maximum of 2.2 mg/g, and inB....
Network Meta-Analysis of Perioperative Analgesic Effects of Different Interventions on Postoperative Pain After Arthroscopic Shoulder Surgery Based on Randomized Controlled Trials
Network Meta-Analysis of Perioperative Analgesic Effects of Different Interventions on Postoperative Pain After Arthroscopic Shoulder Surgery Based on Randomized Controlled Trials
BackgroundShoulder arthroscopic surgery is a common surgical method used in orthopedics. However, severe postoperative pain can significantly limit the early joint movement of pati...
Relationships of BRAF V600E Gene Mutation With Some Immunohistochemical Markers and Recurrence Rate in Patients With Thyroid Carcinoma
Relationships of BRAF V600E Gene Mutation With Some Immunohistochemical Markers and Recurrence Rate in Patients With Thyroid Carcinoma
Background: The B-type rafkinase (BRAF) V600E gene mutation plays an important role in the pathogenesis, diagnosis, and prognosis of thyroid carcinoma. This study was conducted to ...
Mutation in homologous recombination to predict a better prognosis in endometrial cancer.
Mutation in homologous recombination to predict a better prognosis in endometrial cancer.
6082 Background: Endometrial cancers have been categorized into four genomic classes by The Cancer Genome Atlas Research Network (TCGA) with comprehensive genomic analysis. Howeve...
Cervical cancer screening utilization and predictors among eligible women in Ethiopia: A systematic review and meta-analysis
Cervical cancer screening utilization and predictors among eligible women in Ethiopia: A systematic review and meta-analysis
BackgroundDespite a remarkable progress in the reduction of global rate of maternal mortality, cervical cancer has been identified as the leading cause of maternal morbidity and mo...
The Clinical Implications of JAK2V617F Mutation in 137 Chinese Patients with Myeloproliferative Diseases.
The Clinical Implications of JAK2V617F Mutation in 137 Chinese Patients with Myeloproliferative Diseases.
Abstract
A single acquired mutation in the JAK2 gene has recently been described in the classic myeloproliferative diseases(MPDs) including 65–97% of patients with p...