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Protocol for preparing minced Artemia nauplii v1
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Protocol for preparing minced Artemia napulii as feed for young medusa of upside-down jellyfish Cassiopea andromeda Laboratory hatched Artemia nauplii (Instar I & II stage) were filtered using a 250-micron sieve and concentrated to volume. The Artemia nauplii concentrate were centrifuging @ 8000 rpm for 3 minutes. The supernatant was removed and Artemia nauplii biomass was macerated using a sterile plastic pestle. Completely minced Artemia biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL. Two drops ( ) of minced Artemia biomass was fed to young medusa.
Title: Protocol for preparing minced Artemia nauplii v1
Description:
Protocol for preparing minced Artemia napulii as feed for young medusa of upside-down jellyfish Cassiopea andromeda Laboratory hatched Artemia nauplii (Instar I & II stage) were filtered using a 250-micron sieve and concentrated to volume.
The Artemia nauplii concentrate were centrifuging @ 8000 rpm for 3 minutes.
The supernatant was removed and Artemia nauplii biomass was macerated using a sterile plastic pestle.
Completely minced Artemia biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL.
Two drops ( ) of minced Artemia biomass was fed to young medusa.
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