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Squid photoreceptor phospholipase C is stimulated by membrane Gqα but not by soluble Gqα
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Phospholipase C (PLC) was purified from squid retina. Soluble Gqα, membrane Gqα and Gβγ were isolated from GTPγS‐treated and light‐illuminated photoreceptor membranes. The membrane Gqα stimulated phosphatidyl inositoi‐phospholipase C (PI‐PLC) activity in a dose‐dependent manner. Soluble Gqα and membrane Gβγ showed no stimulating effects on PLC. GTPγS‐binding was found exclusively in membrane fraction, with very little present in the KCl‐soluble fraction which contained soluble Gqα. These results indicate that light‐activated rhodopsin activates PLC through membrane‐bound Gqα and suggest that the rhodopsin/Gq/PLC cascade might be the pathway of phototransduction in squid photoreceptors.
Title: Squid photoreceptor phospholipase C is stimulated by membrane Gqα but not by soluble Gqα
Description:
Phospholipase C (PLC) was purified from squid retina.
Soluble Gqα, membrane Gqα and Gβγ were isolated from GTPγS‐treated and light‐illuminated photoreceptor membranes.
The membrane Gqα stimulated phosphatidyl inositoi‐phospholipase C (PI‐PLC) activity in a dose‐dependent manner.
Soluble Gqα and membrane Gβγ showed no stimulating effects on PLC.
GTPγS‐binding was found exclusively in membrane fraction, with very little present in the KCl‐soluble fraction which contained soluble Gqα.
These results indicate that light‐activated rhodopsin activates PLC through membrane‐bound Gqα and suggest that the rhodopsin/Gq/PLC cascade might be the pathway of phototransduction in squid photoreceptors.
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