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The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits
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Abstract
Background
Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation.
Results
TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits.
Conclusions
TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.
Springer Science and Business Media LLC
Title: The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits
Description:
Abstract
Background
Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex.
The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth.
Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation.
Results
TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration.
Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits.
Conclusions
TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.
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