Antimicrobial and Antioxidant Activities of Commercialized Turkish Propolis Extract, and Application to Beef Meatballs

Propolis is a resinous substance produced by bees that is rich with phenolic and flavonoid compounds. Extract of propolis has a high antioxidant and antimicrobial properties due to the presence of these compounds. Therefore, the main objectives of this study were to examine the antibacterial and the antioxidant activities of standardized, commercial water extract of propolis in vivo assays, and to assess its impact on preservation of raw beef meatballs aerobically packaged and stored at 4ºC for 7 days. The results showed that the propolis extract demonstrated the highest antibacterial activity against Staphylococcus epidermidis in vivo test. Furthermore, meatballs prepared with the propolis extract had an extended shelf life about a week in comparison to the regular meatballs. Besides, the propolis extract was a very effective natural antioxidant agent for controlling the oxidative changes in meatballs. The propolis treatment provided 64.6% reduction in the malondialdehyde formation at the final day of storage. Color lightness and yellowness values of meatballs were not affected by the propolis treatment, only difference was observed in redness values. The pH of the meatballs prepared with the propolis extract almost stayed constant during storage, while the pH of the control meatball samples increased. In summary, propolis extract exhibited a strong antimicrobial and antioxidant activity in vivo assays and in a meat product. Accordingly, it should be used in meat product formulations to enhance preservation of meat products.