Comparative study of coagulation effect of Diospyros Malabarica

In most of the Ayurvedic remedies, Diospyros malabarica is used to obtain Rakthasthambana (coagulation) effect. The effect of leaves and stem bark extract of Diospyros malabarica on human blood coagulation was investigated using Lee and White method at Gampaha Wickramarachchi Ayurveda Institute in Sri Lanka. A total of 20 healthy individuals representing both sex were included in the study. For the test group, three tubes containing a volume of 0.5mL of stem bark extract were allocated, and the sap was replaced with normal saline for the tubes of the control group. A volume of 1mL of drawn blood was quickly added for every six tubes in situ, and all were incubated in a water bath at 37˚C. Every tube of two groups was observed carefully for a clot to measure the average clotting time of each group separately. Above procedure was repeated to all the fresh juice samples of stem bark and the leaves of Diospyros malabarica. The statistically significant reduction of average clotting time has proved that the stem bark of Diospyros malabarica and leaves of Diospyros malabarica have a highly significant effect on clotting cascade.