Effect of selenium on reproductive function and oxidative stress in male rats with cisplatin-induced testicular damage

Objective: Cisplatin (Cis) is an anticancer agent used to treat several types of cancers, including testicular cancer. However, despite its therapeutic efficacy, it increases the levels of reactive oxygen species (ROS), which damage healthy tissues and cells, resulting in tissue toxicity. This study aimed to examine the healing effect of selenium (Se) on cisplatin-induced testicular injury in male rats by evaluating testicular weight and monitoring the levels of oxidative stress and reproductive function markers in testicular homogenates. Methods: A total of 38 male rats were divided into four distinct groups. The control group was administered an isotonic solution as the solvent for the active ingredients, while the Se group was administered 1 mg/kg Se dissolved in an isotonic solution via gastric gavage. The Cis group was administered 7.5 mg/kg Cis dissolved in an isotonic solution. The Cis+Se group received both the treatments. Reproductive function and oxidative stress markers in testicular homogenates were analyzed using ELISA. Results: Compared to the control group, the Cis group exhibited significantly lower testicular weight, antioxidant activity (superoxide dismutase [SOD]; total antioxidant status [TAS], P < 0.001), and reproductive function activity (follicle-stimulating hormone [FSH]; Inhibin B, P < 0.001; luteinizing hormone [LH], P < 0.01), and significantly higher oxidant levels (malondialdehyde [MDA], P < 0.01; total oxidant status [TOS], P < 0.01). The Cis+Se group exhibited significantly higher TAS (P < 0.04) and testosterone (P < 0.01) levels than the control group. Conclusion: Administration of Se ameliorated the toxic effects of Cis. Owing to its antioxidant effects, Se may be useful in preventing Cis-induced toxicity in the testicles by alleviating oxidative stress.