Search engine for discovering works of Art, research articles, and books related to Art and Culture
ShareThis
Javascript must be enabled to continue!

Comparative modeling of the H4-H5-loop of the α2-isoform of Na+/K+-ATpase α-subunit in the E1 conformation

View through Europeana Collections
Restraint-based comparative modeling was used for calculation and visualization of the H4-H5-loop of Na+/K+-ATPase from mouse brain (Mus musculus, adult male brain, α2-isoform) between the amino acid residues Cys336 and Arg758 in the E1 conformation The structure consists of two well separated parts. The N-domain is formed by a seven-stranded antiparallel β-sheet with two additional β-strands and five α-helices sandwiching it, the P-domain is composed of a typical Rossman fold. The ATP-binding site was found on the N-domain to be identical in both α2- and α1-isoforms. The phosphorylation Asp369 residue was found in the central part of the P-domain, located at the C-terminal end of the central β-sheet. The distance between the α-carbon of Phe475 at the ATP-binding site and the α-carbon of Asp369 at the phosphorylation site is 3.22 nm. A hydrogen bond between the oxygen atom of Asp369 and the nitrogen atom of Lys690 was clearly detected and assumed to play a key role in maintaining the proper structure of the physphorylaton site in E1 conformation.
Library of the Czech Academy of Sciences
Title: Comparative modeling of the H4-H5-loop of the α2-isoform of Na+/K+-ATpase α-subunit in the E1 conformation
Description:
Restraint-based comparative modeling was used for calculation and visualization of the H4-H5-loop of Na+/K+-ATPase from mouse brain (Mus musculus, adult male brain, α2-isoform) between the amino acid residues Cys336 and Arg758 in the E1 conformation The structure consists of two well separated parts.
The N-domain is formed by a seven-stranded antiparallel β-sheet with two additional β-strands and five α-helices sandwiching it, the P-domain is composed of a typical Rossman fold.
The ATP-binding site was found on the N-domain to be identical in both α2- and α1-isoforms.
The phosphorylation Asp369 residue was found in the central part of the P-domain, located at the C-terminal end of the central β-sheet.
The distance between the α-carbon of Phe475 at the ATP-binding site and the α-carbon of Asp369 at the phosphorylation site is 3.
22 nm.
A hydrogen bond between the oxygen atom of Asp369 and the nitrogen atom of Lys690 was clearly detected and assumed to play a key role in maintaining the proper structure of the physphorylaton site in E1 conformation.

Related Results

Maailmakirjanduse mõõtmisest meil ja mujal / Conceptualizations of World Literature in Estonia and Elsewhere
Maailmakirjanduse mõõtmisest meil ja mujal / Conceptualizations of World Literature in Estonia and Elsewhere
Teesid: Artikkel käsitleb maailmakirjanduse mõiste mahu ja sisu muutumist alates selle esilekerkimisest 19. sajandi algupoolel kuni tänapäeva käsitlusviisideni ja dilemmadeni, mill...
Advanced Liquefaction Cycle for Natural Gas
Advanced Liquefaction Cycle for Natural Gas
This paper presents a LNG Liquefaction cycle configuration using two stages of methane expansion and a single stage of nitrogen expansion (Dual Refrigerant) to improve the efficien...
Loop assembly v2
Loop assembly v2
This protocol is used for performing Type IIS assembly by either BsaI or SapI-mediated restriction/ligation using Loop assembly. Loop assembly comprises8receiver plasmids in odd ...
PO-238 Urinary metabolomics study on the anti-depression effect of different exercise modes on CUMS model rats
PO-238 Urinary metabolomics study on the anti-depression effect of different exercise modes on CUMS model rats
Objective To study the effects of different exercise modes on CUMS depression model rats by 1H-NMR metabolomics technique, and to explore the mechanism of exercise anti-depression ...
Peculiarities of the Arabic Language Processing: Morphological Modeling
Peculiarities of the Arabic Language Processing: Morphological Modeling
The paper deals with the features of morphological modeling of the Arabic language based on the definition of the specifics of its formalization. Morphological modeling is one of t...
Characteristics of carcass and non-carcass components of lambs fed wet brewery waste as a roughage feed
Characteristics of carcass and non-carcass components of lambs fed wet brewery waste as a roughage feed
The objective of the present study was to evaluate the effect of including wet brewery waste (WBW) as the exclusive dietary roughage source on the characteristics of carcass and no...
Semisupervised Deep State-Space Model for Plant Growth Modeling
Semisupervised Deep State-Space Model for Plant Growth Modeling
The optimal control of sugar content and its associated technology is important for producing high-quality crops more stably and efficiently. Model-based reinforcement learning (RL...

Recent Results

Figuring out the facts: calculating mosaic labour times in 4th-c. A.D. Britain
Figuring out the facts: calculating mosaic labour times in 4th-c. A.D. Britain
In the study of the British countryside in the 4th c. A.D. villas have formed a central component. Their decoration features highly in the scholarship, the tessellated floors cited...
Divergent semantic integration (DSI): Extracting creativity from narratives with distributional semantic modeling
Divergent semantic integration (DSI): Extracting creativity from narratives with distributional semantic modeling
AbstractWe developed a novel conceptualization of one component of creativity in narratives by integrating creativity theory and distributional semantics theory. We termed the new ...
Projection-domain iteration to estimate unreliable measurements
Projection-domain iteration to estimate unreliable measurements
AbstractDue to the beam-hardening effect of the broad energy spectrum of the X-ray source in computed tomography, the reconstructed images usually suffer from severe artifacts when...

Back to Top