Effect of Folic Acid and Ascorbic Acid Supplementation on Hematological Parameters of Male Rabbits

Ascorbic acid (Vitamin C) and folic acid (Vitamin B9) are essential micronutrients involved in hematopoiesis, immune function, and antioxidant defense. Ascorbic acid enhances iron absorption and red blood cell (RBC) production, while folic acid is crucial for DNA synthesis and erythropoiesis. Deficiencies in these vitamins are associated with anemia and impaired immune responses. This study investigates the effects of ascorbic acid, folic acid, and their combination on hematological parameters in male rabbits, focusing on hemoglobin (Hb), RBC count, white blood cell (WBC) count, platelets (PLAT), and hematocrit (HCT). Male rabbits were divided into four experimental groups: control, ascorbic acid, folic acid, and a combination of both. Hematological parameters were assessed after treatment, and statistical significance was evaluated. The findings demonstrated that supplementation with ascorbic acid and folic acid significantly improved Hb and RBC counts compared to the control, suggesting enhanced erythropoiesis. The combination group exhibited the highest RBC count (8.60 ± 0.417 ×10⁶/µl), indicating a potential synergistic effect. The WBC count decreased in the folic acid group but remained stable in the ascorbic acid and combination groups, suggesting an immunomodulatory role. Platelet counts increased significantly in the combination group (489.12 ± 209.7 ×10³/µl), highlighting a possible stimulatory effect on megakaryocyte maturation. HCT levels were highest in the combination group (39.66 ± 1.016 ×10³/µl), indicating improved oxygen-carrying capacity.