EFFECT OF PLANT REMEDY «BRAIN-PROPHYT» ON SPATIAL MEMORY IN RATS WITH BRAIN ISCHEMIA

The aim of the research . Determination of the effect of the plant extract «Brain-Profit» on spatial memory in rats using the cerebral ischemia model. Materials and methods . The experiments were conducted on male Wistar rats.The first group consisted of sham-operated rats, the second group consisted of rats with cerebral ischemia, and the third and fourth groups consisted of rats with cerebral ischemia that received «Brain-profit» (100 mg/kg) and standardized Ginkgo biloba extract (50 mg/ kg) (EGb), respectively. The effect of the extract on spatial memory in rats was evaluated using the 8-arm maze method. Pathomorphological studies of rat brain samples were performed using light microscopy. The brain content of bdnf was determined using the ELISA method. The antioxidant status was studied in brain tissue by the activity of antioxidant enzymes – superoxidismutase (SOD), catalase, and the content of lipoperoxidation products – malodialdehyde (MDA). Results . The administration of the extract to rats with brain ischemia had a nootropic effect: the number of errors in reference and working memory was 2,9 and 6,5 times lower, respectively, compared to the control. In the morphological study, the degree of hippocampal damage was less in the experienced rats compared to the control. Administration of «Brain-profit» and Egb to rats increased the content of bdnf in the hippocampus by 48% and 28%, respectively, compared to the control. The SOD and catalase activities were 82% and 36% higher, respectively, and MDA content was 30% and 32% lower, respectively, compared to the control. Conclusion . The «Brain-profit» has a positive effect on spatial memory in rats with cerebral ischemia. The extract increases the content of bdnf in the hippocampus, enhances the antioxidant potential, and prevents neuronal damage. The nootropic effect of the extract is due to the neurotrophic, neuroprotective, and antioxidant actions of the biologically active substances.