Inhibitory effects of trans-cinnamaldehyde on candida spp. Biofilm and planktonic form compared to nystatin: A comparative analysis

Trans-Cinnamaldehyde, an α, β-unsaturated aromatic aldehyde, was a derivative of Cinnamonnum obtusifolium - Lauraceae;  its bioavailability allows it to be used in the treatment of fungal infections as  Candida species causing opportunistic fungal infections are common today. Antibiotic resistance in fungi is often due to their ability to form biofilm. Therefore, new substances with antibiofilm were being  investigated as alternative treatment. Representatives of Candida spp. were used to determine the MIC, MBIC50, MBIC80, MBIC100, and MFC of trans-Cinnamaldehyde compared to Nystatin  which is  used for treatment of Candida infections in Vietnam. The most important antibiofilm effect of trans-Cinnamaldehyde was demonstrated by the CLSI M27-A2 method. Trans-Cinnamaldehyde was given MBIC100 higher than PMIC from 10 to 20 times, while Nystatin was given MBIC100 higher than PMIC from 5 to 20 times. In addition, PMFC of trans-Cinnamaldehyde was equivalent (Candida albicans and Candida tropicalis) or only twice (Candida glabrata) PMIC. The jump in Candida biofilm inhibitory activity was short, from MBIC50 to MBIC100 (no intermediate value, MBIC80). Trans-Cinnamaldehyde extract, compared to Nystatin, had a generally similar effect in preventing the growth and loss of Candida spp. both in biofilm and planktonic form and was somewhat more effective against Candida spp. in biofilm form.