Abstract 1646: Humanized TROP2 mice provide a preclinical tool for the evaluation of therapeutic drugs targeting TROP2

Abstract Human TROP2, also known as tumor-associated calcium signal transducer 2 (TACSTD2), is a type I cell surface glycoprotein that mediates intracellular calcium signals. It plays a crucial role in cell proliferation, survival, self-renewal, and cell adhesion. Several ligands are found to interact with TROP2, including claudin-1, claudin-7, cyclin D1, and IGF-1. TROP2 is expressed on the surface of many stem and progenitor cells as well as other tissues. Importantly, TROP2 is highly expressed in a variety of solid tumors, making it a new target for the development of antibody-conjugated drugs. To evaluate the efficacy of anti-TROP2 antibodies, we generated a humanized B-hTROP2 mouse at Biocytogen. In this mouse model, the murine TROP2 gene was replaced by the full length human TROP2 gene. Western blot analysis showed that human TROP2 protein expression was detected in skin and kidney of homozygous B-hTROP2 mice. These results suggest that B-hTROP2 mice is an ideal tool for in vivo efficacy evaluation of therapeutic drugs targeting human TROP2. Citation Format: Dexuan Huang, Zhiyuan Shen, Limei Ma, Chong Li, Tianquan Jin. Humanized TROP2 mice provide a preclinical tool for the evaluation of therapeutic drugs targeting TROP2 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 1646.