A Novelty Photothermal Therapy of Human Prostate Cancer Pc3 Cell Line Using Multi-Wall Carbon Nanotubes and Platinum Nanocomposite

Multi-wall carbon nanotubes (MWCNTs) coated with platinum nanoparticles (Pt-NPs) was prepared as a novel composite nanomaterials. MWCNTs and Pt-NPs were synthesized using waste synthetic oil, and green chemistry respectively. MWCNT-COOPt, and MWCNT-Pt were characterized using TEM, FE-SEM, AFM, and FT-IR techniques. The diameter of synthesized MWCNTs was between (26.80 - 44.66) nm, while it was between (56.49-89.38) nm for the Pt-NPs. The formation of MWCNT-COOPt and MWCNT-Pt were confirmed using Raman and EDX techniques. The effect of MWCNT-COO, MWCNT-COOPt, and MWCNT-Pt against prostate cancer cell line (PC3) was studied using MTT assay at various concentrations following near-infrared (NIR) irradiation (under λ=1064 nm, P=15.3 W) at different time intervals (30, 60, 90, and 120) sec. The composite of Pt-NPs upon MWCNTs surface enhances its ability to absorb (NIR) radiation and gives rise to an increase in the temperature of cancer cells as a result of plasmon phenomena. These composites were utilized in a novelty treating against human PC3 cell line. The maximum temperatures for MWCNT-COO, MWCNT-COOPt, and MWCNT-Pt were recorded at concentration 25 µg/mL (43.4, 45.8 and 46.2) ˚C, respectively, and irradiation time was recorded at 120 second. These compounds were also found to have a high cytotoxicity towards human PC3 cell line (58.6, 71.6 and 79.6) %, respectively. This study shows that the combination of MWCNTs and Pt-NPs in the photothermal therapy method has given encouraging results to utilize this approach as a local therapy for prostate cancer treatment in a time and concentration dependent manner.