Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Captopril Inhibits Peptidylglycine- α-Hydroxylating Monooxygenase: Implications for Therapeutic Effects
View through CrossRef
The therapeutic actions of captopril are facilitated by its sulfhydryl moiety which interacts with the metal (Zn<sup>2+</sup>) prosthetic groups of angiotensin-converting enzyme (ACE; EC 3.4.15.1). This study focused on captopril as an inhibitor of another metal-dependent (Cu<sup>2+</sup>) enzyme, peptidylglycine-α-hydroxylating monooxygenase (PHM; EC 1.14.17.3). PHM is rate limiting in α-amidation, a COOH-terminal modification that bioactivates several pressor peptides. Captopril inhibited PHM in vitro in a dose-dependent manner with an IC<sub>50</sub> of approximately 100 μmol/l. This inhibition was partially reversed by increased concentrations of Cu<sup>2+</sup>. Structurally similar nonsulfhydryl ACE inhibitors did not affect the activity of PHM. The present findings indicate that the therapeutic effectiveness of captopril may result from actions on a range of metalloenzymes including ACE and PHM.
Title: Captopril Inhibits Peptidylglycine- α-Hydroxylating Monooxygenase: Implications for Therapeutic Effects
Description:
The therapeutic actions of captopril are facilitated by its sulfhydryl moiety which interacts with the metal (Zn<sup>2+</sup>) prosthetic groups of angiotensin-converting enzyme (ACE; EC 3.
4.
15.
1).
This study focused on captopril as an inhibitor of another metal-dependent (Cu<sup>2+</sup>) enzyme, peptidylglycine-α-hydroxylating monooxygenase (PHM; EC 1.
14.
17.
3).
PHM is rate limiting in α-amidation, a COOH-terminal modification that bioactivates several pressor peptides.
Captopril inhibited PHM in vitro in a dose-dependent manner with an IC<sub>50</sub> of approximately 100 μmol/l.
This inhibition was partially reversed by increased concentrations of Cu<sup>2+</sup>.
Structurally similar nonsulfhydryl ACE inhibitors did not affect the activity of PHM.
The present findings indicate that the therapeutic effectiveness of captopril may result from actions on a range of metalloenzymes including ACE and PHM.
Related Results
Aspirin enhances the antihypertensive effect of captopril in spontaneously hypertensive rats.
Aspirin enhances the antihypertensive effect of captopril in spontaneously hypertensive rats.
Activation of renal or vascular prostaglandin mechanisms (or both) has been proposed to contribute to the antihypertensive action of captopril. In conscious spontaneously hypertens...
e0545 Research on inhibiting vascular remodelling of Captopril and Xuefuzhuyu Capsule on the basis of restraining activation and migration of adventitial fibriblasts
e0545 Research on inhibiting vascular remodelling of Captopril and Xuefuzhuyu Capsule on the basis of restraining activation and migration of adventitial fibriblasts
Background
Vascular remodelling is the common pathophysiological basis of artherosclerosis, hypertension and coronary artery disease, et al. It is also one of pat...
Synergistic Effects of Metformin and Captopril on C. elegans
Synergistic Effects of Metformin and Captopril on C. elegans
There are over 400 million patients suffering from type 2 diabetes and over 1 billion suffering from hypertension. Pharmaceuticals commonly used by these patients include Metformi...
Enhancement of EPC migration by high-dose lisinopril is superior compared to captopril and ramipril
Enhancement of EPC migration by high-dose lisinopril is superior compared to captopril and ramipril
Background:
Angiotensin-converting enzyme (ACE) inhibitors have been shown to promote endothelial progenitor cell (EPC) function. However, the efficacies of dif...
Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers
Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers
Ammonia oxidizers (family Nitrobacteraceae) and methanotrophs (family Methylococcaceae) oxidize CO and CH4 to CO2 and NH4+ to NO2-. However, the relative contributions of the two g...
Preformulation studies on the development of a transdermal therapeutic system with captopril
Preformulation studies on the development of a transdermal therapeutic system with captopril
The article presents the results of a study of the process of captopril penetration through a semipermeable membrane by in vitro dialysis to practically confirm the possibility of ...
A Single Membrane Protein Required for Atrial Secretory Granule Formation
A Single Membrane Protein Required for Atrial Secretory Granule Formation
Abstract
The discovery of atrial secretory granules and the natriuretic peptides stored in them identified the atrium as an endocrine organ. Alth...
Mise au point de préparations hospitalières pédiatriques de captopril poudre pour solution orale
Mise au point de préparations hospitalières pédiatriques de captopril poudre pour solution orale
Introduction : Le captopril est indiqué dans le traitement de l'hypertension chronique et l’insuffisance cardiaque chronique chez les enfants. L’absence de forme orale liquide avec...