Abstract 5948: EGR1 Expression and Perineural Invasion in Salivary Duct Carcinoma: A comparison of SDCDN and SDCXPA

Abstract Salivary duct carcinoma (SDC) is primarily categorized as de novo (SDCDN) or ex pleomorphic adenoma (SDCXPA). Previous reports indicate a higher frequency of HMGA2 and PLAG1 fusion events in SDCXPA. Surgical resection remains the main intervention due to limited guidance on new treatment strategies. However, frequent recurrence and challenging management of metastasis highlight the necessity for innovative treatments. This study aimed to investigate SDC characteristics, including perineural invasion (PNI), and elucidate its carcinogenic mechanisms and adverse prognostic factors. We analyzed 52 patients with SDC diagnosed in the National Cancer Center Hospital from 2014 to 2023. To compare gene expression profiles, we performed immunohistochemical staining, including Her2, androgen receptor (AR), PLAG1, and HMGA2, followed by human epidermal growth factor receptor 2 (HER2) in situ hybridization and RNA sequencing of 30 cases. Differential analysis identified genes subjected to immunohistochemical staining and statistical analysis. Based on histologic classification using PLAG1 and HMGA2, 52 cases were classified as 26 cases (50%) SDCDN and 26 cases (50%) SDCXPA. Compared with SDCXPA, SDCDN showed higher perineural, venous, and lymphatic invasion rates (P = .0005.0294, and .0044, respectively). Genetic expression profiling revealed clustering tendencies between these subtypes. Focusing on PNI, gene expression was decreased in early growth response 1 in tumor portions infiltrating perineural tissues, indicating a negative correlation (P < .0001). Similarly, ARID1A expression was elevated in tumor regions of cases with perineural invasion; however, immunohistochemical analysis showed no difference between perineural and non-perineural areas. Furthermore, RNA sequencing identified three novel fusion genes in SDC. In conclusion, clinical disparities between SDCDN and SDCXPA based on molecular and pathological features were observed. We found early growth response 1-brain-derived neurotrophic factor-linking crosstalk between cancer cells and nerves for PNI in SDC, offering insights into future treatment and prognostic factors. Citation Format: Airi Sakyo, Eijitsu Ryo, Seiichi Yoshimoto, Go Omura, Chihiro Fushimi, Toshihiko Sakai, Yoshifumi Matsumoto, Azusa Sakai, Kohtaro Eguchi, Yo Suzuki, Kazuki Yokoyama, Yoshitaka Honma, Yasushi Yatabe, Fumihiko Matsumoto, Taisuke Mori. EGR1 Expression and Perineural Invasion in Salivary Duct Carcinoma: A comparison of SDCDN and SDCXPA [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 5948.