ANTIPROLIFERATIVE EFFECT OF MERREMIA EMARGINATA (burm.F.) LEAF EXTRACT ON SAOS-2 CELL LINE

Introduction: One of the most prevalent malignant tumors is osteosarcoma. The use of herbal medicines that can suppress or kill cancer cells through their antioxidant properties is growing in popularity nowadays as a result of the numerous negative effects of cancer medications. Different parts of M.emarginata have been mentioned to be therapeutically used as diuretic and neuralgia. The main aim of this study is to find out an antiproliferative activity of M.emarginataleaf extract on SAOS 2 cell lineMaterials and Methods: The Merremia emarginata(burm.F.) leaf were soxhlet extracted. Cultured Saos Cells were stimulated with various concentrations of M.emarginata(burm.F.) leaf extracts. To dissolve the formazan formed from MTT, the cells were resuspended in dimethyl sulfoxide (DMSO). The inhibitory rate of cell growth was calculated. mRNA expression levels were examined using real-time PCR.Results: As the concentration of leaf extract increases from 50μg/ml to 200μg/ml the cell viability is significantly decreased. Bcl2 and Bcl-xl expression were significantly negatively correlated with higher concentration of Saos -2 cells treated with M.emarginata.Conclusion: The study revealed that M.emarginatahas a significant role in controlling bone cancer cell proliferation by upregulating caspase 3 mRNA and down regulating the gene expression of Bcl 2 and Bcl xl.