Abstract 5339: Molecular phylogenetic classification of human papillomaviruses and cancer associated genes: In correlation with cervical cancer manifestation

Abstract Human papillomaviruses (HPVs) are a diverse group of small double stranded Deoxyribonucleic acid viruses implicated in many malignancies including cervical cancer. The viruses are also attributed to premalignant epithelial lesions in this disease. Cervical cancer is a leading course of morbidity and mortality in African women and beyond. We aimed to study the molecular phylogenetic classification of HPVs and cervical cancer associated genes in relation with tumour manifestation. Phylogenetic tree was constructed based on nucleotide sequence alignments using ClustalX 2.1 algorithms to classify 31 different HPV DNA virus types and 10 cervical cancer associated genes. Study of immunohistochemistry markers associated with cervical cancer pathology was performed. The nucleotide sequences used include HPV- 1 (V01116), HPV-14 (D90261), HPV-20 (D90262), HPV-21 (D90263), HPV-25 (D90264), HPV-5 (M22961), HPV-47 (M32305), HPV-8 (M12737), HPV-2 (X55964), HPV-57 (X55965), HPV-6: X00203), HPV-11 (M14119), HPV-13 (X62843), HPV-43 (HE962420.1 E6 gene), HPV-44 (U31788.1), HPV-42 (HE820226.1 E6 gene for E6 protein), HPV-16 (K02718), HPV 16 (KJ869425.1 E6 and E7 variants), HPV-35 (M74117), HPV-31 (J04353), HPV-52 (KJ869430.1), HPV-33 (M12732), HPV-58 (D90400), HPV-51 (M62877), HPV-18 (X05015), HPV-45 (M27363), HPV-39 (M38185), (ME180, M73258), HPV 16 (KJ869425.1 E6 and E7 variants), Ki-67 (NM_002417.4), p53 (AB082923.1), EGFR (NM_005228.3), MYC (M25803.1), KRAS (EF471957.1), TGF-B (AF254794.1) and RB (AH002963.2) genes. The resulting phylogenetic tree provides a conventional classification of the HPVs and the cancer genes into groups encompassing the known tissue tropism and oncogenic potential of each gene type associated with the disease. The implications of a phylogenetic taxonomy on the diagnostic detection of HPVs as they relate to cervical cancer associate genes are shown to be important in disease management. Citation Format: John Idoko, Mohammed Faruk, Jigo Yaro, James Enemari, Peter Akpulu. Molecular phylogenetic classification of human papillomaviruses and cancer associated genes: In correlation with cervical cancer manifestation [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 5339. doi:10.1158/1538-7445.AM2017-5339