Phytochemical Screening, Formulation and Optimization of Ehretia Laevis Loaded Silver Nanogel

Abstract Purpose The purpose of this study was to prepare, characterize, and optimize an Silver Nanoparticle loaded nanogel(AgNP) through the ethyl acetate fraction of Ehretia laevis leaf extract through the green synthesis system to heal topical wounds better, particularly problems in permeation and sluggish inflammation in chronic wounds. Method Phytochemical screening was used to verify that the extract had flavonoids, phenolic compounds, and triterpenoids. Green method was used to prepare AgNPs, which were optimized through a 3-level factorial design in order to obtain the desired size of the particle, zeta potential and solubility. UV-Vis spectroscopy, FTIR, LC-MS QTOF (detecting 28 bioactive compounds), FESEM (morphology) and XRD (crystallinity) were used as the methods of characterization. The optimized AgNPs were placed in a carbopol-based gel where they were tested in terms of physicochemical characteristics (pH, viscosity, spreadability), drug release, antimicrobial activity, and antioxidant potential. Results The optimized AgNPs had an average hydrostatic diameter of 103.4 nm, zeta potential of -28.7 mV, a solubility of 0.877 mg/mL, a round shape, crystalline structure, and a 97.54 percent solubility of drug. The nanogel exhibited optimal properties: PH 7, viscosity 3655 cps, spreadability 3.1g.cm/s and drug release 97.54%. The Ehretia laevis phytochemical synergies and AgNPs were found to improve antimicrobial and antioxidant effects. Conclusion The Ehretia laevis AgNP-loaded nanogel is a potentially suitable therapy in topical wound healing because of its better physicochemical, release, and bioactivity characteristics. Efficacy and safety needs additional in vivo and clinical trials to prove.