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Molecular identification and sequencing of Varroa destructor in western Romania
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Varroa destructor mite (Mesostigmata - Varroidae) is one of the major problems of beekeeping worldwide, due to colony mortality at high levels of infestation and transmission off different pathogens between hives. It has been also observed to cause lower weight in infested individuals, decreased learning capacity, and shorter lifespan. The current study was undertaken to character-ize morphologically and molecularly the acarian V destructor, identified in two regions of western Romania. The samples were collected from Arad County, from different localities, namely Nădlac, Roșia Nouă and Obârșia, and from Caraș Severin County, Corneava locality. From a total of 41 samples, collected from 8 different hives, 4 positive samples for Varroa spp. were identified by light microscopy and Scanning Electron Microscopy (SEM) method. Molecular analysis of the Cox1 gene of the mite revealed positive results for all the isolates. The samples were sequenced using next-generation sequencing (NGS) identified as Varroa destructor. This study contributes to the molecular epidemiology of Varroa destructor in Western Romania.
Universitatea de Stiintele Vietii Regele Mihai I din Timisoara
Title: Molecular identification and sequencing of Varroa destructor in western Romania
Description:
Varroa destructor mite (Mesostigmata - Varroidae) is one of the major problems of beekeeping worldwide, due to colony mortality at high levels of infestation and transmission off different pathogens between hives.
It has been also observed to cause lower weight in infested individuals, decreased learning capacity, and shorter lifespan.
The current study was undertaken to character-ize morphologically and molecularly the acarian V destructor, identified in two regions of western Romania.
The samples were collected from Arad County, from different localities, namely Nădlac, Roșia Nouă and Obârșia, and from Caraș Severin County, Corneava locality.
From a total of 41 samples, collected from 8 different hives, 4 positive samples for Varroa spp.
were identified by light microscopy and Scanning Electron Microscopy (SEM) method.
Molecular analysis of the Cox1 gene of the mite revealed positive results for all the isolates.
The samples were sequenced using next-generation sequencing (NGS) identified as Varroa destructor.
This study contributes to the molecular epidemiology of Varroa destructor in Western Romania.
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