Ectomycorrhizal synthesis between four Bolete species and two kinds of trees

Abstract The cultivation of Bolete has very high economic value and ecological value. In order to select suitable symbionts for cultivation, we conducted greenhouse-based mycorrhizal experiments on four boletes(Suillus bovinus, Suillus luteus, Suillus grevillei and Retiboletus sinensis) and two plants(Pinus thunbergii and Quercus acutissima). All plant seedlings were either inoculated or not inoculated with the four Bolete liquid strains. Three months after inoculation, Suillus bovinus, Suillus luteus, Suillus grevillei and Retiboletus sinensis ectomycorrhizae were successfully formed on these two tree species, as evidenced by both morphological and molecular analyses. The mycorrhizal infection rate of all plants reached 40–55%, and the morphology of mycorrhiza is determined by the type of host plant and has nothing to do with the species of Bolete. Subsequently, plant growth, photosynthesis and endogenous hormone secretion were evaluated, mainly affected by host species. The infection of four boletes significantly promoted the growth and photosynthesis rate of host plants, the secretion of IAA, ZT and GA increased, and ABA decreased significantly. In addition, we found IAA in the fermentation broth of four kinds of Bolete. And Retiboletus sinensis can differentiate fruiting bodies by artificial pure culture.