The effect of compression to platelet rich fibrin towards fibroblast proliferation of periodontal ligament: An in vitro study

Objective: The aim of this study was to determine the effect of compression to PRF towards the proliferation of periodontal ligament fibroblasts. Material and Methods: In this study, periodontal ligament fibroblasts were divided into four groups: periodontal ligament fibroblasts, periodontal ligament fibroblasts with platelet rich fibrin non-compressed, periodontal ligament fibroblasts with platelet rich fibrin compressed, periodontal ligament fibroblasts with platelet rich fibrin releasate. Each group consisted of 6 samples. The examination of fibroblast proliferation was done using MTT Assay on day 3 and 7. Results: Results were analyzed using ANOVA test followed by post hoc test with at least significant difference. Based on the result, non-compressed PRF showed the highest fibroblast proliferation. It also showed a significant difference between groups of periodontal ligaments, non-compressed PRF and compressed-PRF. Meanwhile, groups of non-compressed-PRF and PRF-releasate showed insignificant difference. Conclusion: The conclusion of this study showed that non-compressed PRF and PRF releasate is more effective to increase the periodontal ligament proliferation.