Design and Synthesis of Ligand‐Tag Exchangeable Photoaffinity Probe Utilizing Nosyl Chemistry

Construction of a fluorophore or high‐sensitive mass tag on target molecules would promise the facile analysis in photoaffinity labeling. A novel 2‐nitrobenzenesulfonyl (nosyl; Ns) diazirine, which exhibits bifunctional properties for efficient photoaffinity labeling, was designed. This strategy highlights the simplicity of the chemical probe and its ability to install identification tags via Meisenheimer complex by SNAr after photo‐labelling. Herein, in this study, we synthesized new Ns diazirine and demonstrated further studies in terms of photoactivation and SNAr reaction in aqueous condition.